eif4g 2858 antibodies Search Results


eif4g 2858 antibodies  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc eif4g 2858 antibodies
Figure 1. Regulation of eIF4E and cap-dependent translation initiation by 4E-BP1 and <t>eIF4G.</t>
Eif4g 2858 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4g+2858+antibodies/eIF4GI+Antibody/10__1016_slash_j__ejmech__2020__112655-206-49-55
Average 94 stars, based on 1 article reviews
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4e bp1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc 4e bp1
Fig. 1. Regulation of eIF4E and cap-dependent translation initiation <t>by</t> <t>4E-BP1</t> and eIF4G.
4e Bp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4g+2858+antibodies/4E-BP1+Antibody/pm32739551-86-10-19
Average 96 stars, based on 1 article reviews
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resource source identifier antibodies gapdh cell signaling  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc resource source identifier antibodies gapdh cell signaling
Fig. 1. Regulation of eIF4E and cap-dependent translation initiation <t>by</t> <t>4E-BP1</t> and eIF4G.
Resource Source Identifier Antibodies Gapdh Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4g+2858+antibodies/SARS-CoV-2+Nucleocapsid+Protein+Rabbit+mAb/pm35732190-195-2-7
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mouse anti psma4 santa cruz cat  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse anti psma4 santa cruz cat
Fig. 1. Regulation of eIF4E and cap-dependent translation initiation <t>by</t> <t>4E-BP1</t> and eIF4G.
Mouse Anti Psma4 Santa Cruz Cat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4g+2858+antibodies/HSP70/pm35732190-195-49-51
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Image Search Results


Figure 1. Regulation of eIF4E and cap-dependent translation initiation by 4E-BP1 and eIF4G.

Journal: European Journal of Medicinal Chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Figure 1. Regulation of eIF4E and cap-dependent translation initiation by 4E-BP1 and eIF4G.

Article Snippet: Fmoc-Glu(2-OiPrPh) was purchased from Sigma and Fmoc-Lys(Mtt)-OH was purchased from ChemImpex; both were used as received. eIF4E and 4E-BP1 proteins were expressed and purified as previously described.19-20 IC50 values were determined using PPI cat-ELCCA as reported.19 Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques:

Figure 5. Inhibition of the eIF4E−4E-BP1 and eIF4E−eIF4G PPIs in cells as determined via the

Journal: European Journal of Medicinal Chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Figure 5. Inhibition of the eIF4E−4E-BP1 and eIF4E−eIF4G PPIs in cells as determined via the

Article Snippet: Fmoc-Glu(2-OiPrPh) was purchased from Sigma and Fmoc-Lys(Mtt)-OH was purchased from ChemImpex; both were used as received. eIF4E and 4E-BP1 proteins were expressed and purified as previously described.19-20 IC50 values were determined using PPI cat-ELCCA as reported.19 Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Inhibition

Figure 1. Regulation of eIF4E and cap-dependent translation initiation by 4E-BP1 and eIF4G.

Journal: European Journal of Medicinal Chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Figure 1. Regulation of eIF4E and cap-dependent translation initiation by 4E-BP1 and eIF4G.

Article Snippet: Fmoc-Glu(2-OiPrPh) was purchased from Sigma and Fmoc-Lys(Mtt)-OH was purchased from ChemImpex; both were used as received. eIF4E and 4E-BP1 proteins were expressed and purified as previously described.19-20 IC50 values were determined using PPI cat-ELCCA as reported.19 Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques:

Figure 5. Inhibition of the eIF4E-4E-BP1 and eIF4E-eIF4G PPIs in cells as determined via the

Journal: European Journal of Medicinal Chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Figure 5. Inhibition of the eIF4E-4E-BP1 and eIF4E-eIF4G PPIs in cells as determined via the

Article Snippet: Fmoc-Glu(2-OiPrPh) was purchased from Sigma and Fmoc-Lys(Mtt)-OH was purchased from ChemImpex; both were used as received. eIF4E and 4E-BP1 proteins were expressed and purified as previously described.19-20 IC50 values were determined using PPI cat-ELCCA as reported.19 Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Inhibition

Fig. 1. Regulation of eIF4E and cap-dependent translation initiation by 4E-BP1 and eIF4G.

Journal: European journal of medicinal chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions.

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Fig. 1. Regulation of eIF4E and cap-dependent translation initiation by 4E-BP1 and eIF4G.

Article Snippet: Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques:

Fig. 2. 4E-BP1 stapled peptides. (A) Sequences of 4E-BP1, HCS-4E-BP1 and Lac-4E-BP1 peptides. (B) Peptide helicities as determined via CD spectroscopy in 5 mM sodium phosphate buffer, pH 7.4, 25 C. (C) In vitro inhibitory activities determined via PPI cat-ELCCA. (D) Tabulated helicity and IC50 values (presented as 95% confidence intervals) and SPR data.

Journal: European journal of medicinal chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions.

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Fig. 2. 4E-BP1 stapled peptides. (A) Sequences of 4E-BP1, HCS-4E-BP1 and Lac-4E-BP1 peptides. (B) Peptide helicities as determined via CD spectroscopy in 5 mM sodium phosphate buffer, pH 7.4, 25 C. (C) In vitro inhibitory activities determined via PPI cat-ELCCA. (D) Tabulated helicity and IC50 values (presented as 95% confidence intervals) and SPR data.

Article Snippet: Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Circular Dichroism, In Vitro

Fig. 3. eIF4E target engagement measured by the cellular thermal shift assay (CETSA) in HEK293 cell lysate. eIF4E was detected via Western blot. (A) LacA-4E-BP1. (B) LacB- 4E-BP1.

Journal: European journal of medicinal chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions.

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Fig. 3. eIF4E target engagement measured by the cellular thermal shift assay (CETSA) in HEK293 cell lysate. eIF4E was detected via Western blot. (A) LacA-4E-BP1. (B) LacB- 4E-BP1.

Article Snippet: Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Drug discovery, Thermal Shift Assay, Western Blot

Fig. 4. Cell penetration of the Lac-4E-BP1 peptides. (A) CAPA results. (B) Percent HaloTag occupancy following treatment of cells with 0.74 mM peptide. Statistical significance was determined using an unpaired, two-tailed Student’s t tests (***: p ¼ 0.0003).

Journal: European journal of medicinal chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions.

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Fig. 4. Cell penetration of the Lac-4E-BP1 peptides. (A) CAPA results. (B) Percent HaloTag occupancy following treatment of cells with 0.74 mM peptide. Statistical significance was determined using an unpaired, two-tailed Student’s t tests (***: p ¼ 0.0003).

Article Snippet: Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Two Tailed Test

Fig. 5. Inhibition of the eIF4E4E-BP1 and eIF4EeIF4G PPIs in cells as determined via the m7GDP cap pull-down assay. In all cases, cells were treated with peptides dissolved in water for 6 h and eIF4E was used as a normalization control. (A) MDA-MB-231 cells treated with LacA- or LacB-4E-BP1. (B), (C) MCF-7 and T47D cells, respectively, treated with LacB-4E-BP1.

Journal: European journal of medicinal chemistry

Article Title: A cell-penetrant lactam-stapled peptide for targeting eIF4E protein-protein interactions.

doi: 10.1016/j.ejmech.2020.112655

Figure Lengend Snippet: Fig. 5. Inhibition of the eIF4E4E-BP1 and eIF4EeIF4G PPIs in cells as determined via the m7GDP cap pull-down assay. In all cases, cells were treated with peptides dissolved in water for 6 h and eIF4E was used as a normalization control. (A) MDA-MB-231 cells treated with LacA- or LacB-4E-BP1. (B), (C) MCF-7 and T47D cells, respectively, treated with LacB-4E-BP1.

Article Snippet: Chemiluminescence data was collected on a BioTek Cytation3. eIF4E (9742), 4E-BP1 (9644) and eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Inhibition, Pull Down Assay, Control